Study Set Content:
contain 3-9 monosaccharide units.
Oligosaccharides
can contain more than 9 monosaccharide units.
• Polysaccharides
Complex carbohydrates can be broken down into smaller sugar units through a process known a
hydrolysis
If the oxygen on the anomeric carbon of a sugar is not attached to any other structure, that sugar can act as a
reducing agent
If the oxygen on the anomeric carbon of a sugar is not attached to any other structure, that sugar can act as a reducing agent and is termed a
reducing sugar
Reducing sugars include ALL monosaccharides
maltose, lactose;
Reducing sugars include ALL monosaccharides, maltose, lactose; they reduce copper ions in
Benedict’s and Fehling’s
Reducing sugars include ALL monosaccharides, maltose, lactose; they reduce copper ions in Benedict’s and Fehling’s and forms silver metal with
Tollen's
is a non-reducing sugar
Sucrose
can be dissolved freely in water because water is a polar substance,
Monosaccharide and disaccharide
Monosaccharide and disaccharide can be dissolved freely in water because water is a polar substance, while polysaccharide cannot be dissolved easily in water, because, it has
high molecular weight , which give colloidal solutions in water.
is the most common method for the detection of carbohydrates.
Molisch’s Test
is also used for the analysis of carbohydrate, as reducing sugar
Benedict’s Test
Molisch’s test makes the use of
Molisch’s solution
Molisch’s solution (contains α-naphthol in
95% alcohol)
Molisch’s Test is the most common method for the detection of carbohydrates. Molisch’s test makes the use of Molisch’s solution (contains α-naphthol in 95% alcohol) and concentrated
H2SO4.
Reducing sugars consist of a free
aldehyde or ketone group.
Molisch’s test detects the carbohydrate presence, on the basis of
dehydration reaction
Benedict’s test makes the use of Benedict’s solution as a reagent which contains
copper sulfate, sodium citrate, sodium carbonate.
Molisch’s test detects the carbohydrate presence, on the basis of dehydration reaction where the carbohydrate in the sample is dehydrated into aldehyde by the addition of concentrated
H2SO4
