European Journal of Molecular & Clinical Medicine
ISSN 2515-8260 Volume 07, Issue 10, 2020
789
Larger lesions or suspicious malignancies need an incisional biopsy but smaller lesions < 1 cm should
be excised completely.
Tissue for biopsy should becollected carefully from a particular site of the lesion and it is placed in a
wide-mouthed container with 10% buffered formalin for fixation.
If it is a bloody specimen, then it must be washed in saline before placing in the fixative; the fixative
volume should be at least 10 times the volume of the specimen for optimal and rapid fixation.
4
Saline is not an alternative for formalin fixation. Some studies have expressed that if tissue for biopsy
is placed in saline for 1 hr, and then placed in formalin fixative, tissue distortion (cell vacuolization in
basal layer of epithelium and decreased cohesiveness of collagen fibres in the connective
tissue)occurs. Hence diagnosis appears to be problematic.
5
In case of immunofluorescence, two tissue samples are necessary for vesiculobullous lesions and/or
autoimmune disorders:
One in formalin for routine staining and
Other in Michel’s solution for direct immunofluorescence.
6
Container must be compactly sealed and properly labelled with patient’s name, age, gender and site.
In case biopsy has been taken from multiple sites, different bottles are used denoting the site (right or
left sides).
Then specimen should be handed over to an oral pathologist with relevant documents.
STEP 6: CONCLUSION
Dentistry is an art. Earlier diagnosis depends upon the history and clinical features. The dental clinician
should concentrate several causative factors and possible diagnostic factors – where clinician may require
collection of more information and in-depth clinical examination. At certain times, when there is no
correlation between clinical diagnosis and etiological factors or the laboratory results or radiological
investigations, the pathology (biopsy report) issues the final diagnosis.
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Kim S, Christopher L, Bancroft JD, editors. Bancroft’s Theory and Practice of Histological
Techniques. 7th ed. Philadelphia, USA: Elsevier Health Sciences; 2013. p. 81.
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Sengupta S, Prabhat K, Gupta V, Vij H, Vij R, Sharma V. Artefacts produced by normal saline when
used as a holding solution for biopsy tissues in transit. J Maxillofac Oral Surg 2014;13:148-51.
6.
Rosebush MS, Anderson KM, Rawal SY, Mincer HH, Rawal YB. The oral biopsy: Indications,
techniques and special considerations. J Tenn Dent Assoc 2010;90:17-20.