Study Set Content:
1- Question

Blood collection tubes must be drawn in a specific order to avoid cross-contamination of additives between tubes.

Select the Correct Answer:
2- Flashcard

The recommended order of draw for plastic vacutainer tubes is:

1. blood culture bottle (yellow or yellow-black top)

2. coagulation tube (light blue top).

3. non-additive tube (red top)

Click To Flip the Card
3- Flashcard

additive tubes in this order:

 SST (red-gray or gold top) - Contains a gel separator and clot activator.

 Sodium heparin (dark green top)

 PST (light green top). Contains lithium heparin anticoagulant

 EDTA (lavender top)

 ACDA or ACDB (pale yellow top)

 Oxalate/fluoride (light gray top)

NOTE: Tubes with additives must be thoroughly mixed. Erroneous test results may be obtained when the

blood is not thoroughly mixed with the additive.

Click To Flip the Card
4- Flashcard

Why SERUM is preferred over PLASMA?

1. certain substances like lipemia clearing factor or lipoprotein lipase are co-precipitated during clotting.

2. serum is optically clearer

3. there are potential interference with the use of anticoagulants.

Click To Flip the Card
5- Flashcard

Types of serum:

1. Icteric = yellow colored serum due to increase bilirubin pigment

2. Hemolyze = reddish due to rupture of RBC membrane releasing hemoglobin and other red cell components

3. Lipemic = white colored serum due to high fat content

Click To Flip the Card
6- Flashcard

Serious effects of hemolysis:

1. release of RBC contents including water ↑ K,ACP,Zn,Mg,Na

2. direct interference of hgb. with assays involving color reactions.

Click To Flip the Card
7- Flashcard

transport of specimen

1. Specimen must reach the lab. ideally within 45 mins.

2. During transport agitation must be avoided.

3. prevent direct exposure of the specimen to light especially when planning to measure bilirubin.

4. use dark bottles or cover the specimen receptacle with aluminum foil.

5. transport it in ice especially for unstable substances (ammonia, acid phosphatase, blood gases, catecholamines, plasma renin activity).

Click To Flip the Card
8- Flashcard

specimen storage

Specimen must be stored under suitable conditions:

Ideal temp. for storing serum (long term) is rapid freezing in liquid nitrogen ( -800 C).

For short term storage (6 weeks) -200 C.

Click To Flip the Card
9- Flashcard


prevent clotting of whole blood. They are used when plasma is preferred sample

as in the analysis of acid phosphates whose value is very ↑ when serum is used

because of the disintegration of platelet during clotting.

prevent coagulation by either removing calcium needed in the clotting process or

by neutralizing the enzyme thrombin which converts fibrinogen to insoluble fibrin.

Click To Flip the Card
10- Flashcard

Anticoagulant Interference

1. Dilution of plasma

2. Inhibition of plasma enzyme activities

3. ↓ calcium levels

4. interference with electrolytes analysis

Click To Flip the Card
thumb_up_alt Subscribers
layers 10 Items
folder Medicine Category
0 Reviews
Share It Now!