Study Set Content:
Little/no division/ no growth
Lag phase
Exponential logarithmic growth
Log phase
Adjustment, synthesis of materials for cell division
Lag phase
Cell division
Log phase
Cell begins to divide and enter a period of growth or logarithmic increase
Log phase
new =#dying cells
Stationary phase
logarithmic death/ logarithmic decline phase
Death phase
Limitations of nutrients = deplete
Death phase
# of cells dividing equals the # of dying where the growth rate slows
Stationary phase
The number of deaths exceeds the # of new cells formed
Death phase
Uses of visible light as a source of illumination
Compound Light Microscope
Because objects are observed against
a bright background or “bright field,”
the compound light microscope is
sometimes referred to as a
Brightfield Microscope
Uses a device to scatter/blocks light
from the illuminator. Illuminated
objects are seen against a dark
background or “dark field
Darkfield Microscope
Uses a special condenser containing
an annular (ring-shaped) diaphragm)
Organisms are more easily seen
because the light refracted by living
cells is different from the light
refracted by the surrounding
medium.
Phase Contrast Microscope
contain a built-in ultraviolet (UV) light
source. When the UV light strikes
certain dyes and pigments, these
substances emit a longer-wavelength
light, causing them to glow against a
dark background.
Flourescence Microscope
Like phase-contrast uses differences
in refractive indexes to produce
images
Differential Interference Contrast
(DIC) Microscopy
To observe various stained
specimens
Brightfield Microscope
Examine living microorganisms that
are invisible brightfield microscopy
Darkfield Microscope
observe unstained living
microorganisms.
Phase Contrast Microscope
To provide three-dimentional images
Differential Interference Contrast
(DIC) Microscopy
