Study Set Content:
Is based on the measurement of the brightness of light transmitted by suspended particles
Turbidimetry
Determination of sulfate and phosphate ions.
Nephelometry
In these methods, the activity of the biological agent is determined by measuring the turbidity produced in a series of antibiotic dilutions containing a microbiological culture.
Turbidimetry
The greater the turbidity (due to microbial growth), the ____ the activity of the antibiotic.
less
This procedure is also used to standardize the bacterial concentration of the inoculum used in microbial assays.
Turbidimetry
ASSAYS USING TURBIDIMETRY
1.Assay of Antibiotics, Vitamin B5, B12 and B3
2.Chlortetracycline, Doxycycline, Gentamicin
TYPES OF NMR SPECTROSCOPY
1.Continuous-Wave NMR
2.Fourier-Transform NMR
3.Spin-spin Coupling
4.Carbon – 13 NMR
5.Area under Peak
Ions accelerated
Magnet
Magnetic field deflects lightest ions most
Detector
The ionization technique that is currently most frequently - used due to its easy compatibility with high-pressure liquid
Electrospray Ionization (ESI)
